Phosphinothricin-resistant
WebNov 27, 2024 · In the 1980s, the bialaphos resistance ( BAR) gene and its closely related … WebJan 1, 2008 · The use of phosphinothricin resistance as selectable marker for genetic . transformation of grapevine. R. J ARDAK-J AMOUSSI 1), B. B OUAMAMA 1), A. M LIKI 1), A. G HORBEL 1) and G. M. R EUSTLE 2)
Phosphinothricin-resistant
Did you know?
WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis. WebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ...
WebAug 21, 2024 · After 14 days of incubation at 30 °C, carboxin-resistant transformants were screened for phosphinothricin-resistance protein and GUS expression. The transformants expressing opbar were screened on CYM plates with 150 mg/L phosphinothricin. WebOct 23, 2015 · Increasing PPT-resistant gene diversity and generating multi-herbicide …
WebLibertyLink (®) cotton cultivars are engineered for glufosinate resistance by … WebJan 18, 2024 · One of the microbes that produce phosphinothricin, Streptomyces hygroscopicus, has an enzyme that inactivates phosphinothricin and glufosinate by acylating it. The gene (bar) encoding this...
WebA bioassay was developed for identifying transgenic wheat plants based on De Block et al.'s [8] ammonium-multiwell assay which allows qualitative and quantitative evaluation of the expression of the enzyme phosphinothricin acetyl transferase (PAT). Important parameters in the assay are the use of young leaf tissues, short incubation period (6 h) and a high …
WebWe have previously transformed rice (Oyrza sativa L.) with the maize ribosome-inactivating protein b-32 gene (Zmcrip3a) and the phosphinothricin resistance gene (bar). In the present study, Southern blot hybridization analysis of 56 primary fertile transformants resulted in distinct band patterns, indicating that all the transformants had been generated by … bebe 2 mois 37 6 temperatureWebJan 1, 2008 · Phosphinothricin resistance as selectable marker for genetic transformation … bebe 2 mois 37 5 temperatureWebPhosphinothricin C5H11NO4P- CID 25244585 - structure, chemical names, physical and … disfraz vaca mujerWebNov 1, 1996 · v ol. 62, 1996 resistance to phosphinothricin in c. reinhardtii 3835 at concentrations as low as 0.27 mM (50 m g/ml), at which both GS activity and ammonium utilization were completely abol- bebe 2 mois 9h sans mangerWebresistance of expiants to, phosphinothricin is presented. Key words: Ammonium - Carbon source - Glufosinate - Glutamine - Metabolic activity - Phosphinothricin Introduction Selectable marker genes are still a necessity in plant trans formation technology. In the presence of a selective agent, only those cells that are transformed and express the bebe 2 mois 37 7 temperatureWebA gene which confers resistance to the herbicide bialaphos (bar) has been characterized. The bar gene was originally cloned from Streptomyces hygroscopicus, an organism which produces the tripeptide bialaphos as a secondary metabolite. Bialaphos contains phosphinothricin, an analogue of glutamate which is an inhibitor of glutamine synthetase. bebe 2 ansWebSep 12, 2015 · In this study, we successfully performed Agrobacterium-mediated genetic transformation of Salvia miltiorrhiza and produced herbicide-resistant transformants. Leaf discs of S. miltiorrhiza were infected with Agrobacterium tumefaciens EHA105 harboring pCAMBIA 3301. The pCAMBIA 3301 includes an intron-containing gus reporter and a bar … bebe 2 meses y medio