Clontech y1h
WebThe yeast one-hybrid (Y1H) system is a powerful tool for the identification and isolation of cDNAs of transcription factors using promoter segments or regulatory elements as baits. Here we propose an adaptation of the Y1H system for identification and cloning of transcription factors using Matchmaker (Clontech) Y2H cDNA libraries. http://www.labbase.net/Supply/SupplyItems-4229347.html
Clontech y1h
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WebAug 25, 2016 · Yeast one-hybrid (Y1H) assay has been proven to be a powerful technique to characterize in vivo the interaction between a given transcription factor (TF), or its DNA-binding domain (DBD), and target DNA sequences. ... Purified pHISi (Clontech) vector (300 ng/μL). 3. EcoRI and XbaI, restriction enzymes. 4. Gel Extraction Kit. 5. T4 polymerase ... WebClontech - enzyme digestion, linking and recombination in vivo Invitrogen - gateway method; Electrotransformation of recombinant products to yeast competent cells Clontech - cDNA Invitrogen - mixed plasmids of secondary library; Library detection and plasmid extraction ; Library screening process of prokaryotic yeast one-hybrid (Y1H)
WebUSD $185.00. Matchmaker Insert Check PCR Mix 1 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, dNTPs and buffer; simply add cells to the mix … WebOct 16, 2014 · Candidate genes identified by yeast one-hybrid (Y1H) screening ... (PT3530-5, Clontech), and the pre-prepared Y187 yeast competent cells. Transformants that grow in selective medium (S.D./-
Web丁香通为您找到145条dna疫苗性腺整合信息，包括dna疫苗性腺整合报价行情，优质供应商，图片，品牌等最新信息，丁香通为买家提供用户服务，诚信保障等服务，批发采购dna疫苗性腺整合,上丁香通。 WebI used the Matchmaker Gold yeast one-hybrid system from Clontech. ... The p53 fragment was from pGBKT7-p53 used for Y2H. I don’t know whether this fragment is suitable for Y1H.
Web菌落PCR产品，验证整合到Y1H酵母基因组的bait DNA序列 . 品牌：Clontech 技术资料：0 篇 【商品总览】Clontech【630495】酵母双杂培养基套装（含 ...
WebApr 30, 2024 · To further ascertain whether the specific binding of PavCYP78A9 was directly regulated by PavAGL15 in sweet cherry fruit, point-to-point Y1H experiments were performed to detect the interactions between PavAGL15 and the PavCYP78A9 promoter. The PavAGL15 protein directly bound to the promoter sequence of PavCYP78A9 in vitro … for what it\u0027s worth meaning phraseWebSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA and protein sequences. Annotate features on your plasmids using the curated feature database. Store, search, and share your sequences, files and maps. for what it\u0027s worth meaning spanishWebAug 14, 2015 · zt487 Y1H Gold Clontech 酵母单杂交系统. zt488 pGADT7-Rec Clontech 酵母双杂交系统. zt489 pX334 Addgene CRISPR/Cas 系统. zt490 pX335 Addgene CRISPR/Cas 系统. zt491 pCas-Gidue Origene CRISPR/Cas 系统. zt492 pShuttle-IRES-hrGFP2 Stratagene 腺病毒系统 directions to patricias petalsWebClontech Laboratories, Inc. www.clontech.com Protocol No. PT3529–1 A Takara Bio Company Version No. PR7Z2445 7 Matchmaker™ One-Hybrid Library Construction & … for what it\u0027s worth - movieWebFeb 22, 2006 · Y1H reporter strains were prepared by cloning three to four tandem repeats of: 1) the cis-elements specific for two classes of homeodomain transcription factors (HDZipI-II) [23, 24]; 2) the Drought Responsive Element (DRE) from the promoter of the rd29A gene from Arabidopsis thaliana, specific for the DREB/CBF subfamily of single … directions to peabody ksWebNov 27, 2024 · Cloning of transcription factors (TFs) We employed the Yeast One-Hybrid assay (Y1H) (Clontech Y1H, Clontech, USA) to identify TFs that interact with the cloned … for what it\u0027s worth remakeWebApr 1, 2016 · CLONTECH公司酵母单杂交试剂盒说明书.pdf.pdf. 2016-04-01上传. 暂无简介 for what it\u0027s worth quote